Osmo-Sensitive and Stretch-Activated Calcium-Permeable Channels in Vicia faba Guard Cells Are Regulated by Actin Dynamics

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Osmo-sensitive and stretch-activated calcium-permeable channels in Vicia faba guard cells are regulated by actin dynamics.

In responses to a number of environmental stimuli, changes of cytoplasmic [Ca(2+)](cyt) in stomatal guard cells play important roles in regulation of stomatal movements. In this study, the osmo-sensitive and stretch-activated (SA) Ca(2+) channels in the plasma membrane of Vicia faba guard cells are identified, and their regulation by osmotic changes and actin dynamics are characterized. The ide...

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Actin dynamics regulates voltage-dependent calcium-permeable channels of the Vicia faba guard cell plasma membrane.

Free cytosolic Ca(2+) ([Ca(2+)](cyt)) is an ubiquitous second messenger in plant cell signaling, and [Ca(2+)](cyt) elevation is associated with Ca(2+)-permeable channels in the plasma membrane and endomembranes regulated by a wide range of stimuli. However, knowledge regarding Ca(2+) channels and their regulation remains limited in planta. A type of voltage-dependent Ca(2+)-permeable channel wa...

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External pH effects on the depolarization-activated K channels in guard cell protoplasts of Vicia faba

Previous studies reveal that the pH of the apoplastic solution in the guard cell walls may vary between 7.2 and 5.1 in closed and open stomata, respectively. During these aperture and pH changes, massive K+ fluxes cross the cellular plasma membrane driving the osmotic turgor and volume changes of guard cells. Therefore, we examined the effect of extracellular pH on the depolarization-activated ...

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K+ transport properties of K+ channels in the plasma membrane of Vicia faba guard cells

Electrical properties of the plasma membrane of guard cell protoplasts isolated from stomates of Vicia faba leaves were studied by application of the whole-cell configuration of the patch-clamp technique. The two types of K+ currents that have recently been identified in guard cells may allow efflux of K+ during stomatal closing, and uptake of K+ during stomatal opening (Schroeder et al., 1987)...

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Deltamethrin rescues run down of K+ outward rectifying channels in Vicia faba guard cells

The dominant outward rectifier K+ currents were examined in protoplasts from Vicia faba guard cells. In whole-cell patch-clamp recordings, we generally observed that the conductance of the K+ inward and the outward rectifier gradually decreases. As a consequence of this run down a new steady state was achieved which was 90 ± 5% lower than that obtained at the beginning of the recording. The run...

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ژورنال

عنوان ژورنال: Plant Physiology

سال: 2007

ISSN: 0032-0889,1532-2548

DOI: 10.1104/pp.106.091405